Bioinformatics Databases
As part of our services, the GACRC builds and hosts local copies of frequently cited application data, and provides assistance for sharing data among GACRC members. Datasets are located in the commonly shared "/db" filesystem. NCBI BLAST datasets are pre-formatted to work with BLAST and BLAST+ and are located in "/db/ncbiblast/" and are organized by date.
NCBI BLAST Datasets can be loaded in the same way as software modules. This allows users to replicate results by always being able to use a time stamped version of a database. The time stamp corresponds to the download date of the dataset. To search for available NCBI BLAST dataset modules run the command:
ml spider ncbiblastdb
You can then load a database by running the command module load. For example to load the databases timestamped at 05/02/2025:
module load ncbiblastdb/20250502
Loading this module sets the environment variable BLASTDB to /db/ncbiblast/20250502. This allows BLAST+ to search that directory for databases. You can then use the name of the database you would like to use.(nt, nr, etc.) Here is an example of a shell script to run BLAST+ on the batch queue using the database nt:
#!/bin/bash
#SBATCH --job-name=j_BLAST+
#SBATCH --partition=batch
#SBATCH --mail-type=ALL
#SBATCH --mail-user=username@uga.edu
#SBATCH --ntasks=1
#SBATCH --mem=10gb
#SBATCH --time=08:00:00
#SBATCH --output=BLAST+.%j.out
#SBATCH --error=BLAST+.%j.err
cd $SLURM_SUBMIT_DIR
module load BLAST+/2.16.0-gompi-2024a
module load ncbiblastdb/20250502
blastn -query example.fasta -out results.out -db nt
In your actual submission script, use your own discretion for the Slurm header values.
NCBI's nr and nt datasets are the most commonly used at the GACRC. GACRC staff will update these datasets every 3 months as well as other NCBI BLAST datasets: cdd_delta, human_genome, mouse_genome, nrte, refseq_protein, refseq_rna, swissprot, and taxdb datasets.
Various subject datasets, e.g. pfam, bowtie indexes of human and mouse, and NCBI bacterial datasets, are hosted as well. However, these datasets are not accessed with a module. These datasets either don’t need updating, the source does not update frequently, or the datasets are not used frequently by our users. These datasets will only be updated by user request. Instead of using a module, to use these databases, use the path to the databases in your script. Below is an example using BLAST+ with a dataset not available as a module.
#!/bin/bash
#SBATCH --job-name=j_BLAST+
#SBATCH --partition=batch
#SBATCH --mail-type=ALL
#SBATCH --mail-user=username@uga.edu
#SBATCH --ntasks=1
#SBATCH --mem=10gb
#SBATCH --time=08:00:00
#SBATCH --output=BLAST+.%j.out
#SBATCH --error=BLAST+.%j.err
cd $SLURM_SUBMIT_DIR
module load BLAST+/2.13.0-gompi-2022a
blastn -query example.fasta -out results.out -db /db/ncbiblast/refseq_microbial/fasta/09162020/
For datasets requested by individual lab groups, GACRC encourages users to maintain their own copies of databases, whose files can be installed in their group's shared "/work" area. Similarly for datasets frequently updated by their source, the GACRC encourages users to maintain their own copies of these public databases.
The set of data which are regularly updated is open to review, and can be expanded based on available GACRC resources.
Installed Bioinformatics Databases
| Name | Latest Date (Version) | Module Available |
|---|---|---|
| AlphaFold | 09/03/2023 (v2.3.4) | no |
| AlphaFold3 | 01/14/2025 ("20241114") | no |
| AlphaPulldown | 02/28/2025 (v2.0.0) | no |
| bakta | 05/08/2025 ("20250224") | no |
| beav | 09/19/2024 (v1.4.0) | no |
| bowtie2 | 09/12/2024 | no |
| Bracken | 08/27/2025 | no |
| checkv | 01/10/2023 (v1.5) | no |
| DRAM_data | 03/12/2026 | no |
| eggnog-mapper | 05/05/2025 (v2.1.12) | no |
| EnTAP | 06/09/2025 (v2.2.0) | no |
| EUKulele | 04/15/2025 ("phylodb") | no |
| FA-nf | 04/09/2025 (v0.4.0) | no |
| foldseek | 08/08/2025 | no |
| funannotate | 01/28/2025 (v1.8.17) | no |
| GAMD | 04/17/2025 ("20220215") | no |
| GTDB-Tk | 05/16/2025 (release226) | no |
| GToTree | 11/25/2025 | no |
| GetOrganelle | 01/13/2022 | no |
| humann | 03/26/2023 (v3.6/v3.9) | no |
| InterProScan | 04/07/2025 (v5.55-88.0) | no |
| Kraken2 (PlusPF, PlusPFP, PlusPFP-16, core_nt, EuPathDB, GTDB) | 08/14/2025 | no |
| MaarjAM | 11/20/2024 | no |
| MetaMaps | 11/03/2021 (v0.1) | no |
| metawrap | 01/30/2026 | no |
| NCBI BLAST Databases | First week of Feb., May, Aug., & Nov. | yes |
| NCBI-FCS | 12/01/2023 (v0.5.0) | no |
| pfam | 12/18/2021 (v34.0) | no |
| PHASTEST | 02/01/2024 | no |
| QIIME (SILVA v138.2) | 10/20/2025 | no |
| repbase | 02/03/2025 (v28.12) | no |
| RoseTTAFold | 11/20/2024 (v1.0.0) | no |
| sortmerna | 03/04/2016 | no |
| trinotate | 10/16/2023 | no |
| Uniprot | 11/21/2024 ("20230615") | no |
| Uniref | 03/31/2026 | no |
| VFDB | 06/30/2025 | no |
| VirSorter | 11/12/2020 ("20230609") | no |
| virulencefinder | 05/06/2024 | no |