Bioinformatics Databases

As part of our services, the GACRC builds and hosts local copies of frequently cited application data, and provides assistance for sharing data among GACRC members. Datasets are located in the commonly shared "/db" filesystem. NCBI BLAST datasets are pre-formatted to work with BLAST and BLAST+ and are located in "/db/ncbiblast/" and are organized by date.

NCBI BLAST Datasets can be loaded in the same way as software modules. This allows users to replicate results by always being able to use a time stamped version of a database. The time stamp corresponds to the download date of the dataset. To search for available NCBI BLAST dataset modules run the command:

ml spider ncbiblastdb

You can then load a database by running the command module load. For example to load the databases timestamped at 06/16/2021:

module load ncbiblastdb/20210616

Loading this module sets the environment variable BLASTDB to /db/ncbiblast/20210616. This allows BLAST+ to search that directory for databases. You can then use the name of the database you would like to use.(nt, nr, etc.) Here is an example of a shell script to run BLAST+ on the batch queue using the database nt:

#!/bin/bash
#SBATCH --job-name=j_BLAST+
#SBATCH --partition=batch
#SBATCH --mail-type=ALL
#SBATCH --mail-user=username@uga.edu
#SBATCH --ntasks=1
#SBATCH --mem=10gb
#SBATCH --time=08:00:00
#SBATCH --output=BLAST+.%j.out
#SBATCH --error=BLAST+.%j.err

cd $SLURM_SUBMIT_DIR
module load BLAST+/2.9.0-gompi-2019b
module load ncbiblastdb/20210616
blastn -query example.fasta -out results.out -db nt

In your actual submission script, use your own discretion for the Slurm header values.

NCBI's nr and nt datasets are the most commonly used at the GACRC. GACRC staff will update these datasets every month as well as other NCBI BLAST datasets: cdd_delta, human_genome, mouse_genome, nrte, refseq_protein, refseq_rna, swissprot, and taxdb datasets.

Various subject datasets, e.g. pfam, bowtie indexes of human and mouse, and NCBI bacterial datasets, are hosted as well. However, these datasets are not accessed with a module. These datasets either don’t need updating, the source does not update frequently, or the datasets are not used frequently by our users. These datasets will only be updated by user request. Instead of using a module, to use these databases, use the path to the databases in your script. Below is an example using BLAST+ with a dataset not available as a module.

#!/bin/bash
#SBATCH --job-name=j_BLAST+
#SBATCH --partition=batch
#SBATCH --mail-type=ALL
#SBATCH --mail-user=username@uga.edu
#SBATCH --ntasks=1
#SBATCH --mem=10gb
#SBATCH --time=08:00:00
#SBATCH --output=BLAST+.%j.out
#SBATCH --error=BLAST+.%j.err

cd $SLURM_SUBMIT_DIR
module load BLAST+/2.9.0-gompi-2019b
blastn -query example.fasta -out results.out -db /db/ncbiblast/refseq_microbial/fasta/09162020/

For datasets requested by individual lab groups, GACRC encourages users to maintain their own copies of databases, whose files can be installed in their group's shared "/work" area. Similarly for datasets frequently updated by their source, the GACRC encourages users to maintain their own copies of these public databases.

The set of data which are regularly updated is open to review, and can be expanded based on available GACRC resources.

Installed Bioinformatics Databases

Name	Version	Module Available
akblab	01/28/2018	no
Bacteria NCBI	12/21/2017	no
hg19,mm10 & mm9	07/29/2016	no
cellranger	10/29/2019	no
conspred_ressources	02/02/2017	no
dammit	03/18/2021	no
deconseq	12/01/2016	no
decontaMiner	08/29/2019	no
eggnog-mapper	04/16/2019	no
funannotate	08/15/2019	no
DTDB-Tk	01/15/2021	no
hg	09/20/2016	no
kegg	09/09/2016	no
maker	05/22/2018	no
MetaCLADE	08/29/2019	no
NCBI BLAST Databases	beginning of every month	yes
NCBI Fasta	beginning of every month	yes
ngs	11/03/2016	no
nndb	04/29/2020	no
PB	04/24/2019	no
pfam	10/07/2021	no
pylosift	07/20/2020	no
Refseq_genomic		no
repbase	01/09/2017	no
rfam	02/09/2017	no
seqdb	05/23/2019	no
sortmerna	02/02/2021	no
TaxDB	02/26/2019	no
topcons2	02/18/2021	no
Uniprot	11/19/2020	no
Uniref	06/28/2018	no
wublast	03/01/2020	no