SOAPdenovo2-Teaching
Category
Bioinformatics
Program On
Teaching
Version
r240
Author / Distributor
Description
"SOAPdenovo is a novel short-read assembly method that can build a de novo draft assembly for human-sized genomes. The program is specially designed to assemble Illumina short reads. It creates new opportunities for building reference sequences and carrying out accurate analyses of unexplored genomes in a cost effective way. SOAPdenovo2 is the successor of SOAPdenovo." More details are at SOAPdenovo2
Running Program
The last version of this application is at /usr/local/apps/eb/SOAPdenovo2/r240-foss-2016b
To use this version, please load the module with
ml SOAPdenovo2/r240-foss-2016b
Here is an example of a shell script, sub.sh, to run on the batch queue:
#!/bin/bash
#SBATCH --job-name=j_SOAPdenovo2
#SBATCH --partition=batch
#SBATCH --mail-type=ALL
#SBATCH --mail-user=username@uga.edu
#SBATCH --ntasks=1
#SBATCH --mem=10gb
#SBATCH --time=08:00:00
#SBATCH --output=SOAPdenovo2.%j.out
#SBATCH --error=SOAPdenovo2.%j.err
cd $SLURM_SUBMIT_DIR
ml SOAPdenovo2/r240-foss-2016b
SOAPdenovo-127mer [options]
In the real submission script, at least all the above underlined values need to be reviewed or to be replaced by the proper values.
Please refer to Running_Jobs_on_the_teaching_cluster, Run X window Jobs and Run interactive Jobs for more details of running jobs at Teaching cluster.
Here is an example of job submission command:
sbatch ./sub.sh
Documentation
ml SOAPdenovo2/r240-foss-2016b
SOAPdenovo-127mer --help
Version 2.04: released on July 13th, 2012
Compile Dec 1 2017 22:19:06
Usage: SOAPdenovo <command> [option]
pregraph construct kmer-graph
sparse_pregraph construct sparse kmer-graph
contig eliminate errors and output contigs
map map reads to contigs
scaff construct scaffolds
all do pregraph-contig-map-scaff in turn
Installation
Source code is obtained from SOAPdenovo2
System
64-bit Linux