CellRanger-ATAC-Sapelo2

Category

Bioinformatics

Program On

Sapelo2

Version

2.1.0

Author / Distributor

Please see https://support.10xgenomics.com/single-cell-atac/software/pipelines/latest/what-is-cell-ranger-atac

Description

"Cell Ranger ATAC is a set of analysis pipelines that process Chromium Single Cell ATAC data. Cell Ranger ATAC includes four pipelines relevant to single cell chromatin accessibility experiments." More details are at https://support.10xgenomics.com/single-cell-atac/software/pipelines/latest/what-is-cell-ranger-atac

Running Program

• Version 2.1.0 is installed at /apps/eb/CellRanger-ATAC/2.1.0

To use version 2.1.0, please load the module with

ml CellRanger-ATAC/2.1.0

Here is an example of a shell script, sub.sh, to run on the batch queue:

#!/bin/bash
#SBATCH --job-name=j_cellranger-atac
#SBATCH --partition=batch
#SBATCH --ntasks=1
#SBATCH --cpus-per-task=8
#SBATCH --constraint=EDR
#SBATCH --mem=32gb
#SBATCH --time=120:00:00
#SBATCH --output=log.%j.out
#SBATCH --error=log.%j.err
#SBATCH --mail-user=username@uga.edu
#SBATCH --mail-type=ALL

cd $SLURM_SUBMIT_DIR
ml CellRanger-ATAC/2.1.0
cellranger-atac count --localcores=8 [options]

In the real submission script, at least all the above underlined values need to be reviewed or to be replaced by the proper values.

Please use --constraint=EDR header in your job submission script for a quicker job start time and optimal job performance.

Here is an example of job submission command:

sbatch ./sub.sh 

Documentation

ml CellRanger-ATAC/1.2.0
cellranger-atac -h

cellranger-atac -h (1.2.0)
Copyright (c) 2019 10x Genomics, Inc.  All rights reserved.
-------------------------------------------------------------------------------

Usage:
    cellranger-atac mkfastq

    cellranger-atac count
    cellranger-atac aggr
    cellranger-atac reanalyze

    cellranger-atac mkref

    cellranger-atac testrun
    cellranger-atac upload
    cellranger-atac sitecheck


cellranger-atac count -h

cellranger-atac count (1.2.0)
Copyright (c) 2019 10x Genomics, Inc.  All rights reserved.
-------------------------------------------------------------------------------

The commands below should be preceded by 'cellranger-atac':

Usage:
    count
        --id=ID
        --fastqs=PATH
        [--sample=PREFIX]
        [options]
    count <run_id> <mro> [options]
    count -h | --help | --version

Arguments:
    id      A unique run id, used to name output folder [a-zA-Z0-9_-]+.
    fastqs  Path of folder created by mkfastq or bcl2fastq.
    sample  Prefix of the filenames of FASTQs to select.

Options:
# Sample Specification
    --reference=PATH	Path of folder containing a 10x-compatible reference.
    			    Required.
    --description=TEXT  More detailed sample description. Optional.
    --lanes=NUMS        Comma-separated lane numbers.
    --indices=INDICES   Deprecated. Not needed with the output of
    			cellranger-atac mkfastq, or bcl2fastq
    --project=TEXT      Name of the project folder within a mkfastq or
                            bcl2fastq-generated folder to pick FASTQs from.
# ATAC analysis 
    --force-cells=N     Define the top N barcodes with the most reads as
                            cells. N must be a positive integer <=
                            20,000. Please consult the documentation
                            before using this option. Optional.
    --dim-reduce=MODE   Dimensionality reduction mode for clustering: 'lsa'
                            (default), 'plsa', or 'pca'. Optional.
# Downsampling
    --downsample=GB     Downsample input FASTQs to approximately GB 
                            gigabases of input sequence. Optional.
# Martian Runtime
    --jobmode=MODE      Job manager to use. Valid options:
                            local (default), sge, lsf, or a .template file
    --localcores=NUM    Set max cores the pipeline may request at one time.
                            Only applies to local jobs.
    --localmem=NUM      Set max GB the pipeline may request at one time.
                            Only applies to local jobs.
    --localvmem=NUM     Set max virtual address space in GB for the pipeline.
                            Only applies to local jobs.
    --mempercore=NUM    Reserve enough threads for each job to ensure enough
                        memory will be available, assuming each core on your
                        cluster has at least this much memory available.
                            Only applies in cluster jobmodes.
    --maxjobs=NUM       Set max jobs submitted to cluster at one time.
                            Only applies in cluster jobmodes.
    --jobinterval=NUM   Set delay between submitting jobs to cluster, in ms.
                            Only applies in cluster jobmodes.
    --overrides=PATH    The path to a JSON file that specifies stage-level
                            overrides for cores and memory.  Finer-grained
                            than --localcores, --mempercore and --localmem.
                            Consult the 10x support website for an example
                            override file.
    --uiport=PORT       Serve web UI at http://localhost:PORT
    --disable-ui        Do not serve the UI.
    --noexit            Keep web UI running after pipestance completes or fails.
    --nopreflight       Skip preflight checks.

    -h --help           Show this message.
    --version           Show version.

Note: 'cellranger-atac count' works as follows:
set --fastqs to the folder containing FASTQ files. In addition,
set --sample to the name prefixed to the FASTQ files comprising your sample. 
For example, if your FASTQs are named:
    subject1_S1_L001_R1_001.fastq.gz
then set --sample=subject1

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Installation

Source code is download from https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/latest

System

64-bit Linux