Maker-Teaching: Difference between revisions
No edit summary |
No edit summary |
||
| Line 24: | Line 24: | ||
=== Running Program === | === Running Program === | ||
The last version of this application is at /usr/local/apps/ | The last version of this application is at /usr/local/apps/gb/maker/2.31.9_conda | ||
To use this version, please load the module with | To use this version, please load the module with | ||
<pre class="gscript"> | <pre class="gscript"> | ||
ml | ml Miniconda3/4.4.10 | ||
</pre> | </pre> | ||
Latest revision as of 09:36, 26 November 2018
Category
Bioinformatics
Program On
Teaching
Version
2.31.9
Author / Distributor
Description
More details from http://gmod.org/wiki/MAKER_Tutorial#What_does_MAKER_do.3F
MAKER is an easy-to-use genome annotation pipeline designed to be usable by small research groups with little bioinformatics experience; however, MAKER is also designed to be scalable and is appropriate for projects of any size even including use by large sequence centers. MAKER can be used for de novo annotation of newly sequenced genomes, for updating existing annotations to reflect new evidence, or just to combine annotations, evidence, and quality control statistics for use in other GMOD programs like GBrowse, JBrowse, Chado, and Apollo.
Running Program
The last version of this application is at /usr/local/apps/gb/maker/2.31.9_conda
To use this version, please load the module with
ml Miniconda3/4.4.10
It is installed at /usr/local/apps/gb/maker/2.31.9_conda via Conda. The sample data and sample control files are at /usr/local/apps/gb/maker/2.31.9_conda/data
Example of the conda version script sub.sh to run on at queue:
#!/bin/bash
#SBATCH --job-name=j_PAML
#SBATCH --partition=batch
#SBATCH --mail-type=ALL
#SBATCH --mail-user=username@uga.edu
#SBATCH --ntasks=1
#SBATCH --mem=10gb
#SBATCH --time=08:00:00
#SBATCH --output=PAML.%j.out
#SBATCH --error=PAML.%j.err
cd $SLURM_SUBMIT_DIR
ml Miniconda3/4.4.10
. /usr/local/apps/eb/Miniconda3/4.4.10/etc/profile.d/conda.sh
conda activate /usr/local/apps/gb/maker/2.31.9_conda
maker -CTL
cp /usr/local/apps/gb/maker/2.31.9_conda/data/maker_exe.ctl /usr/local/apps/gb/maker/2.31.9_conda/data/maker_opts.ctl .
maker [options] <maker_opts> <maker_bopts> <maker_exe>
conda deactivate
In the real submission script, at least all the above underlined values need to be reviewed or to be replaced by the proper values.
Please refer to Running_Jobs_on_the_teaching_cluster, Run X window Jobs and Run interactive Jobs for more details of running jobs at Teaching cluster.
Here is an example of job submission command:
sbatch ./sub.sh
Documentation
ml Miniconda3/4.4.10
conda activate /usr/local/apps/gb/maker/2.31.9_conda
maker -h
MAKER version 2.31.9
Usage:
maker [options] <maker_opts> <maker_bopts> <maker_exe>
Description:
MAKER is a program that produces gene annotations in GFF3 format using
evidence such as EST alignments and protein homology. MAKER can be used to
produce gene annotations for new genomes as well as update annotations
from existing genome databases.
The three input arguments are control files that specify how MAKER should
behave. All options for MAKER should be set in the control files, but a
few can also be set on the command line. Command line options provide a
convenient machanism to override commonly altered control file values.
MAKER will automatically search for the control files in the current
working directory if they are not specified on the command line.
Input files listed in the control options files must be in fasta format
unless otherwise specified. Please see MAKER documentation to learn more
about control file configuration. MAKER will automatically try and
locate the user control files in the current working directory if these
arguments are not supplied when initializing MAKER.
It is important to note that MAKER does not try and recalculated data that
it has already calculated. For example, if you run an analysis twice on
the same dataset you will notice that MAKER does not rerun any of the
BLAST analyses, but instead uses the blast analyses stored from the
previous run. To force MAKER to rerun all analyses, use the -f flag.
MAKER also supports parallelization via MPI on computer clusters. Just
launch MAKER via mpiexec (i.e. mpiexec -n 40 maker). MPI support must be
configured during the MAKER installation process for this to work though
Options:
-genome|g <file> Overrides the genome file path in the control files
-RM_off|R Turns all repeat masking options off.
-datastore/ Forcably turn on/off MAKER's two deep directory
nodatastore structure for output. Always on by default.
-old_struct Use the old directory styles (MAKER 2.26 and lower)
-base <string> Set the base name MAKER uses to save output files.
MAKER uses the input genome file name by default.
-tries|t <integer> Run contigs up to the specified number of tries.
-cpus|c <integer> Tells how many cpus to use for BLAST analysis.
Note: this is for BLAST and not for MPI!
-force|f Forces MAKER to delete old files before running again.
This will require all blast analyses to be rerun.
-again|a recaculate all annotations and output files even if no
settings have changed. Does not delete old analyses.
-quiet|q Regular quiet. Only a handlful of status messages.
-qq Even more quiet. There are no status messages.
-dsindex Quickly generate datastore index file. Note that this
will not check if run settings have changed on contigs
-nolock Turn off file locks. May be usful on some file systems,
but can cause race conditions if running in parallel.
-TMP Specify temporary directory to use.
-CTL Generate empty control files in the current directory.
-OPTS Generates just the maker_opts.ctl file.
-BOPTS Generates just the maker_bopts.ctl file.
-EXE Generates just the maker_exe.ctl file.
-MWAS <option> Easy way to control mwas_server for web-based GUI
options: STOP
START
RESTART
-version Prints the MAKER version.
-help|? Prints this usage statement.
Installation
Conda
System
64-bit Linux